Nouvelles exclusivités
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Exclusivités > Diaclone
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MULTIPLE ANALYTE DETECTION BY FLOW CYTOMETRY
Diaclone DIAplex is a sensitive multiplex fluorescent bead-based immunoassay for the simultaneous quantification of multiple analytes from a single sample by flow cytometry.
Utilising bead populations with distinct fluorescence intensities and unique antibody specificities Diaclone DIAplex can accurately measure multiple analytes in a single sample with a significantly reduced assay time and sample volume requirement when compared to traditional ELISA techniques.
- Antibodies highly specific to the analytes to be detected are coupled to fluorescently labelled detection beads. Each bead is analogous to an individually coated well in an ELISA providing a capture surface for a specific analyte.
- One (simplex) or more (multiplex) populations of the beads are combined in suspension with the sample or standard under test.Any analytes present in the sample will bind to the specific antibodies linked to the fluorescent beads.
- A biotin-conjugated secondary antibody mixture is then added which binds to any analytes captured by the primary antibodies.
- Streptavidin-Phycoerythrin (PE) is added, which binds to the biotin conjugates and emits a yellow fluorescent signal.
- The unique size and fluorescent signature of each bead is differentiated by flow cytometry.
- Following sample data acquisition using a flow cytometer, analyte concentration in the test sample is calculated simply by using the Diaclone DIAplex Pro analysis software provided free of charge.
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FLEXIBLE BY DESIGN
Diaclone DIAplex Complete
DIAplex Complete provides multiplex assay sets which are preconfigured for the detection of a panel of analytes for investigation of a specific scientific area.
An individual experiment can provide measurements of several analytes from a single sample.
Diaclone DIAplex Design
DIAplex Design combines the benefits of multiplexing with the ability to design your own analyte detection panel. The assays have been designed for use individually (simplex) or combined to create any size flex system. Each combination is supplied with a DIAplex accessory kit.
VERSATILITY & SIMPLICITY
- Test multiple sample types including serum, plasma and cell culture supernatants
- Compatible with most commercially available flow cytometers
- The simple to use Diaclone DIAplex Pro software (available free of charge) enables calculation of analyte concentration from a standard curve & provision of detailed analysis reports within a matter of minutes post flow cytometry measurement
EFFICIENCY & RELIABILITY
- Diaclone DIAplex assay standards are calibrated against NIBSC standards
- Robust Validation for uncompromised reproducibility and sensitivity
- Small sample volume required due to multiple analyte detection in one sample maximising the number of analytes that can be tested in a single sample
- A single set of diluted standards is used to generate a standard curve for each analyte
- Takes less time than a conventional ELISA and provides data that would normally require multiple ELISA in a smaller sample volume
With over 20 years experience and extensive expertise, we are committed to providing excellence in Monoclonal Antibody and Immunoassay development. The expanding range of Diaclone immunology products is specifically designed to advance diagnostic and research applications.
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Diaclone DIAplex, Human Th1/Th2/Inflammation
Multiple analyte detection by flow cytometry
Different populations of CD4+ T-Cells secrete differing patterns of cytokines that can lead
to differing immune responses. The T Helper Type-1 (Th1) or Type-2 (Th2) cell cytokines can
direct an antigenic response towards either a cell mediated or a humoral immune response
respectively. The Th1 and Th2 cytokines cross regulate each other providing a mechanism for
regulation of immune responses. A new subset of T helper cells that predominantly produce
IL-17 (Th17 cells) are believed to be the major cell type involved in the induction of various
pro-inflammatory cytokines.
FLEXIBLE BY DESIGN
DIAplex Complete provides multiplex assay sets which are preconfigured for the detection of
a panel of analytes for investigation of a specific scientific area. An individual experiment can
provide measurements of several analytes from a single sample.
DIAplex Design combines the benefits of multiplexing with the ability to design your own analyte
detection panel. The assays have been designed for use individually (simplex) or combined to
create any size flex system. Each combination is supplied with a DIAplex accessory kit.
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Diaclone DIAplex PRO SOFTWARE
The Diaclone DIAplex Pro software is a simple to use tool enabling complete analysis of data files in
minimal time after assay completion. Sample analyte concentrations are automatically calculated
by comparison to a standard curve which is generated by the software and a comprehensive
analysis report generated. Software and instructions for use available free of charge.
DIAplex HUMAN Th1 / Th2 / INFLAMMATION SPECIFICATIONS
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Saviez vous que pour vos recherches en immunologie AbCys et Diaclone vous proposent plus de 100 Immuno-essais ?
ELISA, ELISpot, Flurospot et paires d’anticorps de hautes qualités sont à votre disposition.
Nos ELISA vous permettront de quantifier des molécules de l’apoptose, des molécules d’adhésions, les marqueurs de cellules endothéliales, les cytokines et facteurs de croissance, et bien plus.
Nos ELISA sont disponibles pour l’homme, la souris ou le rat.
Découvrez également notre large choix d'ELISPOT.
Nos kits ont été développés pour offrir un maximum de commodité à l’utilisateur. De plus, tous les anticorps monoclonaux des kits peuvent être obtenus séparément sous forme de paires d'anticorps.
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Il est très facile de vous dire que tous nos kits sont d’une qualité exceptionnelle, c’est pour cela qu’AbCys et Diaclone vous donne la possibilité d’en juger par vous même.
Tous nos Immuno-essais sont disponibles sous un format échantillon à tarif spécial.
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En nouveauté ce mois ci chez AbCys l'ELISA et L’ELISPOT IL-17-A
N'attendez plus !
Vous ne pouvez plus vous tromper.
Maintenant c’est facile de commander les produits de la gamme Diaclone directement chez AbCys, distributeur exclusif !
Demandez le nouveau catalogue de la gamme Diaclone.
Pour toutes informations complémentaires contacter directement Manuel Sacha, business development manager chez AbCys !
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