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Domaines d'activités
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Immunologie > Endocrinologie / Diabète
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  He should be tested for Diabetes !!
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Insulin, a polypeptide hormone with a molecular weight of 5800 Da, is secreted by the beta cells of the islets of Langerhans from the pancreas. Insulin possesses a wide spectrum of biological actions. It stimulates cellular glucose uptake, glucose oxidation, glycogenesis, lipogenesis, proteogenesis and the formation of DNA and RNA. Insulin plays a key role in the regulation of plasma glucose levels (hepatic output inhibition, stimulation of peripheral glucose utilisation).
Applications
- Determination of the beta-cell reserve during glucose tolerance test or after a carbohydrate rich meal, as a guide for the instauration of insulin therapy;
- Contribution to the diagnosis of insulin and non-insulin-dependent diabetes;
- Characterisation and follow-up of states of glucose intolerance;
- Diagnosis and study of cases of insulin resistance;
- Diagnosis of insulinoma and other causes of hypoglycemia.
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Devis |
| BL-28-E |
Insulin
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kit |
96 puits |
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High quality Animal insulin ELISA kits,
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We are providing ordinary assay kits and high specific assay kits for insulin(S Type).
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Devis |
| RSCYK060R |
Insulin Multispecies ELISA
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kit |
96 puits/kit |
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| RSHAKRIN011TR |
Mouse Insulin (TMB), ELISA
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kit |
96 puits/kit |
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| RSHAKRIN010TR |
Rat Insulin (TMB), ELISA
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kit |
96 puits/kit |
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| RSHAKRIN011HR |
Mouse Insulin (H-type), ELISA
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kit |
96 puits/kit |
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| RSHAKRIN010HR |
Rat Insulin (H-Type), ELISA
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kit |
96 puits/kit |
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| RSHAKRIN012TR |
Dog Insulin, ELISA
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kit |
96 puits/kit |
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S-type ELISA has a very low cross-reactivity to proinsulin.
We tried a recovery test by adding proinsulin to assay samples. The assay results are shown below. It proved that proinsulin added to the samples only slightly increased the insulin assay value. This means we can measure insulin precisely in the presence of proinsulin.
Recovery test of proinsulin to rat serum sample n=2
| Rat proinsulin added ng/ml |
Assay value as insulin ng/ml |
Influence assay value/added (%) |
| 0 |
1.00 |
- |
| 1.0 |
1.01 |
1.0 |
| 2.0 |
1.03 |
1.5 |
| 4.0 |
1.07 |
1.8 |
| 8.0 |
1.19 |
2.4 |
Recovery test of proinsulin to mouse serum sample n=2
| Rat proinsulin added ng/ml |
Assay value as insulin ng/ml |
Influence assay value/added (%) |
| 0 |
106.0 |
- |
| 100 |
106.9 |
0.9 |
| 500 |
112.5 |
1.3 |
| 5000 |
286.0 |
3.6 |
Comparison of assay values obtained by ordinary kit (T) and specific kit (S) with rat and mouse serum samples.
As shown in the figures, very high correlation coefficients were obtained with both rat(R=0.9946) and mouse(R=0.9868) samples.
The slopes of the regression lines were 1.209 in rat and 1.198 in mouse. This means that type S kits gave about 20% lower assay values than ordinary T-type kits with both rat and mouse samples. This must be caused by improvement of specificity in S-type. Though we should be careful in thinking that the difference is directly related proinsulin, difference of 20% is a reasonable value of proinsulin ratio to IRI in human subjects.
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Prix |
Devis |
| RSHAKRIN011SR |
Mouse Insulin (S-type), ELISA
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kit |
96 puits/kit |
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| RSHAKRIN010SR |
Rat Insulin (S-type), ELISA
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kit |
96 puits/kit |
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Proinsulin (PI), a polypeptide of 9390 MW (86 amino acids) is synthetized in the ß cells of the Islets of Langerhans in the pancreas. The level of proinsulin in serum can be a reflection of marker cell status and a consequence of disfunction of PI processing and/or secretion. Proinsulin like material is increased in clinical conditions as insulinoma, familial hyperinsulinemia, non-insulin dependent diabetes mellitus.
Applications
- A 100% reliable marker for an advanced marker ß Cell dysfonction
- early markers for diabetes type 2 (Earlier than the blood sugar values)
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Dénomination |
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Fiche |
Prix |
Devis |
| RD193094100 |
Proinsulin Intact ELISA
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kit |
96 puits |
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Insulin is synthesized in the beta-cells of the islets of Langerhans as a precursor molecule, proinsulin. In the secretory granules of the beta-cells, proinsulin is cleaved into insulin and into a 31-amino?acid peptide, called the Connecting Peptide or C?Peptide. Insulin and C-Peptide are secreted in equimolar amounts. However, because of its longer half-life, the plasma concentration of C-peptide is higher than that of insulin.
The determination of plasma C-Peptide allows an assessment of the endogenous insulin production, even in the presence of exogenous insulin administration or in the presence of circulating anti?insulin antibodies
Applications
- Assessment of residual beta-cell function in diabetics under insulin therapy
- Detection and monitoring of the remission phase of type I diabetes
- Adjunct in the differential diagnosis between type I (insulin-dependent) and type II (non insulin-dependent) diabetes
- Diagnosis of insulin-induced factitious hypoglycaemia
- Contribution to the diagnosis of insulinoma (insulin suppression test)
- Prognostic index of foetal outcome in pregnant diabetic women
- Evaluation of insulin secretion in liver disease
- Monitoring of pancreatectomy
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| Code |
Dénomination |
Clone |
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Condi. |
Inf. |
Fiche |
Prix |
Devis |
| BL-30-E |
C-PEP
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kit |
96 puits |
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Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.
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Fiche |
Prix |
Devis |
| RD194062400 |
Acylated Ghrelin
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kit |
96 puits |
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| RD394062400 |
Acylated Ghrelin
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kit |
96 puits |
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| RD194063400 |
Unacylated Ghrelin
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kit |
96 puits |
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| RD394063400 |
Unacylated Ghrelin
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kit |
96 puits |
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Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. . In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.
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| RD191001100 |
Leptin ELISA Kit, Clinical Range
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kit |
1 x 96 puits |
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| RD291001200 |
Leptin ELISA Kit, Clinical Range
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kit |
1 x 96 puits |
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Clusterin (Apolippoprotein J; SP-40,40; TRPM-2; SGP-2; pADHC-9; CLJ; T64; GP III; XIP8) is a highly conserved disulfide-linked secreted heterodimeric glycoprotein of 75-80 kDa but truncated forms targeted to nucleus have also been identified. Recent study demonstrate, that serum Clusterin level increases significantly in diabetic type II patients and in patients with developing coronary heart disease, or myocardial infarction. These date raise the possibility that elevated Clusterin levels in serum may represent a strong indication of vascular damage.
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| RD391034200 |
Clusterin ELISA
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kit |
96 puits |
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Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells.
Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. Of particular interest is that low adiponectin serum levels predict type 2 diabetes independent of other risk factors.
Adiponectin has the potential to become a clinically relevant parameter to be measured routinely in subjects at risk for type 2 diabetes, atherosclerosis and the metabolic syndrome
Applications
- Early indicator for diabetes type of 2
- reliable marker for an insulin resistance
- markers for a successful resistance therapy
- risk marker for coronary heart illnesses
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Prix |
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| RD191023100 |
ADIPONECTINE, high sensitive
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kit |
96 puits |
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| RD293023100 |
Adiponectin ELISA
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kit |
96 puits/kit |
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EGFP-conjugated GLUT1 ligand
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GLUT1 is the most widely expressed isoform of known glucose transporters and the primary glucose transporter across the blood-brain barrier. EGFP-GLUT1 ligand has been shown to bind the carboxy terminal extracellular loop of GLUT1. EGFP-GLUT1 ligand allows GLUT1 expression to be monitored at the plasma membrane of all mammalian cell lines by FACS analyses or fluorescence staining. EGFP-GLUT1 ligand can provide insights, as a qualitative and quantitative marker, into the molecular bases of both normal disordered glucose homeostasis.
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| Code |
Dénomination |
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Type |
Condi. |
Inf. |
Fiche |
Prix |
Devis |
| AbC0118-1 |
GLUT-1 Ligand
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Ag |
200µl / 50 tests |
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 Les Kits de dosage ELISA pour :
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 Hormones et stéroïdes
Progestérone
Testostérone
Cortisol
Oestradiol,...
Eicosanoïdes
Leucotriènes
Prostaglandines
Thromboxane A2...
AZT
Créatine
Isoprostane
Histamine
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Avantages des Kits ELISA Neogen :
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Rapides
Facile à utiliser
De Haute Sensibilité
Pour de Petits Volumes d'échantillons
Pour toutes espèces
Non radioactifs
Reproductibles
Idéal pour l'Automatisation
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La seule façon de détecter le domaine extracellulaire du récepteur au glucose GLUT1 sur les cellules de mammifères
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Quantification de l’activité métabolique lié au glucose grâce à un peptide marqué pour la détection des récepteurs GLUT-1 : EGFP-GLUT1 ligand
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EGFP- GLUT1 ligand
Description:
Quantification de l'activité métabolique liée au glucose grâce à un petide marqué pour la détection des récepteurs Glut-1 : EGFP-GLUT1 ligand
Le transport du Glucose, médié par un ou plusieurs membre des transporteurs du glucose (GLUT) joue un rôle essentiel dans le métabolisme des cellules normales ou pathologiques.
GLUT-1 est l'isoforme le plus largement exprimé des transporteurs du glucose connus et est le principal transporteur du glucose à travers la barrière hémato-encéphalique.
Les forts niveaux d'expression de GLUT-1 dans les cellules tumorales sont associés aux mauvais pronostics d'évolution de différents types de cancers, dont les cancers du sein et de l'estomac (2).
Des recherches récentes montrent que GLUT-1 effectue le transport à travers la barrière hémato-encéphalique de certaines molécules neuro-actives comme les peptides glycosylés.
Il a été démontré que le peptide produit par AbCys : EGFP-GLUT-1 ligand se lie à la partie carboxy-terminale de la boucle extracellulaire de GLUT-1
(contrairement à la plupart des anticorps existants, qui reconnaissent la totalité de la molécule(3)
Ce peptide permet de mesurer l'expression de GLUT-1 sur les membranes des cellules de mammifères par FACS ou marquage fluorescent. (4) et peut procurer des renseignements, en atnt que marqueur qualitatif et quantitatif sur base moléculaire de l'homéostasie normale ou pathologique du glucose, comme le diabète ou comme deux désordres génétiques , le syndrome Fanconi-Bickel ou le syndrome de la maladie De-Vivo(6) .
La détermination de l'expression de GLUT-1 par la liaison du ligand est corrélée à l'augmentation de l'absorption du glucose observée dans les cellules malignes et les cellules au métabolisme actif.
EGFP- GLUT-1 ligand a permis de mesurer l'expression de GLUT -1 pendant l'activation de lymphocytes T et a été déterminant pour l'identification d'une population de thymocytes immatures CD4+ CD8 + possèdant une forte activité métabolique. (7)
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Publications:
1. Hruz PW, Mueckler MM. (2001), Mol Membr Biol., 18:183-93.
2. Kang SS et al. (2002), Jpn J Cancer Res., 93:1123-8.
3. Kawamura et al. (2001), Cancer 92:634-41.
4. Guo X, Geng M, Du G. (2005), Biochem Genet., 43:175-87.
5. Manel N, Kim FJ, Kinet S, Taylor N, Sitbon M, Battini JL. (2003), Cell., 115:449-59.
6. Kim FJ, Manel N, Garrido EN, Valle C, Sitbon M, Battini JL. (2004), Retrovirology, 1:41
7. Manel N, Battini JL, Sitbon M. (2005), J Biol Chem (280):29025-9
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Figure 1: Detection of cell surface GLUT1 using the EGFPGLUT1 ligand.
Human embryonic kidney 293T cells and human erythrocytes (RBC) were incubated for 30 min at 30°C with a 1:25 dilution of EGFP-GLUT1 ligand. Staining profiles were analyzed by FACS and histograms depicting staining with « mock » protein are indicated.
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Figure 2 : Cell surface expression of GLUT1 during T cell activation.
GLUT1 was detected as an early marker of human T cell activation using the EGFP-GLUT1 ligand.
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Figure 3 : GLUT1 accumulation at plasma membrane and cell-to-cell junctions (arrow) of human breast carcinoma cells using the EGFP-GLUT1 ligand.
Primary mammary carcinoma cells were incubated with EGFP-GLUT1 ligand and examined by confocal immunofluorescence microscopy.
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| Code |
Dénomination |
Clone |
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Inf. |
Fiche |
Prix |
Devis |
| AbC0118-1 |
GLUT-1 Ligand
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Ag |
200µl / 50 tests |
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