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Cell Separation > Cell Separation
Unique density gradient separation of lymphocytes from whole blood or biological fluids.
- Ready-to-use, sterile.
- Safe method, minimum contact with biological fluids.
- Time saver, quick and easy sample filling.
- Maximum yield of viable mononuclear cells.
- A large number of samples may be handled at the same time.
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A reply for each application:
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From blood:
| Type of tube |
Code |
Diluted blood (1: 1) |
Undiluted blood |
Tube |
| UNI-SEP |
U-02 (U-03*) |
4 - 8 ml |
2 - 4 ml |
15 ml tube with 2 ml of lymphoprep |
| UNI-SEP+ |
U-04 (U-05*) |
4 - 11 ml |
2 - 5.5 ml |
15 ml tube with 3 ml of lymphoprep |
| UNI-SEP MAXI |
U-10 (U-11*) |
20 - 35 ml |
10 - 17.5 ml |
50 ml tube with 10 ml of lymphoprep |
| UNI-SEP MAXI+ |
U-16 (U-17*) |
Non applicable |
18.5 - 25 ml |
50 ml tube with 15 ml of lymphoprep |
*Tubes provided empty, separation medium to be filled by user.
For bone marrow, buffy coat ( enriched white cell fraction ), lymph and spinal fluid : biological fluids having low red cell contetnt:
| Diluted bone marrwo (1 :1) |
BUFFY BOOSTER * |
Tube |
Code |
| 4 - 8 ml |
0.7 - 1.5 ml |
15 ml tube with 2 ml of lymphoprep |
U-02 |
| 4 - 11 ml |
1 - 2 ml |
15 ml tube with 3 ml of lymphoprep |
U-04 |
| 20 - 35 ml |
3 - 5 ml |
50 ml tube with 10 ml of lymphoprep |
U-10 |
* Add the BUFFY BOOSTER (code U-15) immediately before adding the sample.
1. UNI-SEP products are sterile and ready for use. Open only under aseptic conditions. Best results are obtained when all steps are performed at 18-20°C.
2. Use anticoagulant treated or defibrinated blood. Blood may be diluted with an equal volume of sterile saline or other sterile isotonic buffer or may used undiluted. Add diluted or undiluted blood according to the table1 directly to the tube, cap and centrifuge (18-20°C) 1000 x g for 20 min. Erythrocytes dead cells and PMNs are found at the bottom of the tube. The uni-sep insert separtes the lymphocytes interface from the pellet of packed erythrocytes.
3. Remove the platelet-rich plasma and discard it.
4. Remove the mononuclear layer with the aid of a pipette. Alternatively, the entire contents of the tube above the plastic inset may be removed by decanting the solultion.
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