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Immunology > Oxidative Sress Markers
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Oxidative Sress Markers
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1 - 8OHdG ELISA kit (8hydroxy2'deoxyguanosine)
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Formation of 8-hydroxy-2'-deoxyguanosine(8-OHdG) by oxigen radicals. H Kasai Environmental Mutagen Research,
Vol 10, p73-78(1988)
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Oxidative stress is known to play an important role in the development of various diseases and aging process.
8hydroxy2'deoxyguanosine (8OHdG) is formed when DNA is oxidatively damaged by reactive oxygen species (ROS).
8OHdG is one of the most sensitive biomarker for oxidative stress and can be detected in urine, serum, tissue DNA from human and animals.
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1 High specificity & high sensitivity:
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Cross reactivity was checked for 19 analogues.
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2 Easy operation & speedy:
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There is no need for expensive equipment and sample pretreatment. Required equipments are: pipettes, incubator and micro plate reader with 450nm filter. Assays can be completed within 3.5 hours (New 8OHdG Check).
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New 8OHdG Check ELISA (96wells) code: Coming soon...
(Measuring range: 0.5 200 ng/mL, suitable for urine sample.)
New 8OHdG Check Trial Package (32wells) code: Coming soon...
(Measuring range: 0.5 to 200 ng/mL, suitable for urine sample.)
Highly Sensitive 8OHdG Check ELISA (96 wells) code: Coming soon...
Measuring range: 0.125 to 10 ng/mL, suitable for serum* and tissue DNA.*)
*: sample pretreatment is required.
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2 - Anti 8OHdG monoclonal antibody (clone N45.1)
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Highly specific for 8OHdG.
Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment stained with N45.1.
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Anti 8OHdG mouse monoclonal antibody (IgG1k), lyophilized powder. Cross reactivity was checked for 19 analogues. dA, dC, dT, 2'dG, 8OHdA, 2'dI, 2'dU, O6methyl dG, G, 7methylG, 8BrG, 8sulfhydrylG, 4SHG, 8OHG, Gua, O6methylGua, 8OHGua, uric acid, urea, creatinine, creatine (G: Guanosine, Gua: Guanine)
Anti 8OHdG monoclonal antibody code: Coming soon...
(20 micro gram)
Anti 8OHdG monoclonal antibody code: Coming soon...
(100 micro gram)
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3 - Anti Thymidine Glycol (TG) monoclonal antibody
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Biomarker for oxidative DNA damage
Thymidine glycol (TG) is produced when thymidine is damaged by hydroxy radical, and is a sensitive biomarker for oxidative stress. Anti TG monoclonal antibody specific for TG in DNA polymers. Suitable for immunohistochemical research.
Anti TG monoclonal antibody code: Coming soon...
(100 micro gram)
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4 - Anti 4HNE monoclonal antibody (HNEJ2)
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Highly specific for 4HNE.
Immunohistochemistry of colorectal carcinoma cells. Adenocarcinoma (arrows?) and nontumorous epithelial cells (star?;×240).
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Membrane lipids are one of the targets of reactive oxygen species (ROS).4hydroxy 2nonenal (4HNE) is a major membrane lipid peroxidation product. Monoclonal antibody against 4HNE (clone HNEJ2) is highly specific for 4HNEHis/Lys/Cys adducts. Anti 4HNE mouse monoclonal antibody (IgG1?), lyophilized powder.
For immunohistochemistry and Western blotting
Anti 4HNE monoclonal antibody code: Coming soon...
(20 micro gram)
Anti 4HNE monoclonal antibody code: Coming soon...
(100 micro gram)
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5 - HexanoylLysine adduct (HEL) ELISA kit & antibody
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A new biomarker of lipid peroxidation.
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HexanoylLysine adduct (HEL) is a novel lipid hydroperoxidemodified lysine residues. HEL is formed by oxidative modification by oxidized ω6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation, and is detected in oxidatively modified LDL, in human atherosclerotic lesions and human urine.
ELISA kit suitable for urine and serum samples.
HexanoylLys adduct (HEL) ELISA code: Coming soon...
(96wells)
Suitable for human urine and animal serum. Required equipments are: pipettes, incubator and microplate reader with 450nm filter.
For immunohistochemistry and western blotting.
Anti HEL monoclonal antibody code: Coming soon...
(20 micro gram)
Anti HEL mouse monoclonal antibody (IgG, clone 5H4), lyophilized powder. Cross reactivity was checked for 8 analogues: MDA, glyoxal, methyl glyoxal, 1hexanal, 2hexenal, 1nonannal, 2nonenal and 4hydroxy2nonenal.
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6 - Anti MDA monoclonal antibody (1F83)
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Highly specific for Malondialdehyde (MDA).
Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
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Malondialdehyde (MDA) is one of the major aldehyde derived from lipid peroxidation. This antibody is specific for MDAmodifird proteins, and suitable for immunohistocheistry and western blotting.
For immunohistochemistry and Western blotting.
Anti MDA monoclonal antibody code: Coming soon...
(30 micro gram)
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7 - Acrolein (ACR) ELISA kit & antibody
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Biomarker of lipid peroxidation.
Acrolein (ACR) is a representative carcinogenc aldehyde found ubiquitously in the environment and endogenenouslythrough oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein.
ELISA kit suitable for human urine.
Acrolein (ACR) ELISA code: Coming soon...
(96wells)
For immunohistochemistry and western blotting.
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Anti ACR mouse monoclonal antibody (IgG, clone 5F6). Cross reactivity was checked for 14 analogues: crotonal, 2hexenal, 2octanal, 2 nonenal, 4decanal, 2,4decadional, MDA, 4HHE, 4HPE, 4HON, 4HNE, npropanal, npentanal and nhexenal.
Anti ACR monoclonal antibody code: Coming soon...
(20 micro gram)
Anti ACR monoclonal antibody code: Coming soon...
(100 micro gram)
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8 - Test kit for Total Antioxidant Capacity (PAO)
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Significance of Antioxidants.
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For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention. In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu ++ ⇒ Cu + ), antioxidant capacity of samples can be detected in 5 minutes.
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Application to foods, beverages and serum.
PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
Potential Anti Oxidant (PAO) code: Coming soon...
(96wells)
Suitable for human and animal serum.
Required equipments are: pipettes, and microplate reader with 492nm filter.
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