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Immunology > Detection Systems
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AbCys : The distributor of VECTOR Laboratories since 1999
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Developing innovative labeling and detection systems for biological and medical science.
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We propose to you quality reagents for :
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- Immunohistochemistry
- Immunofluorescence
- Neuronal Tracing
- DNA/RNA Labeling
- In Situ Hybridization
- Blotting Applications
- Chemiluminescence
- ELISAs
- Flow Cytomerty
 ImmPRESS Kit
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The ImmPRESS polymerized reporter enzyme staining system is based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme "micropolymers" avoids the intrinsic shortcomings of using large dextrans or other macromolecules as backbones. Attaching a unique "micropolymer" with a high density of very active enzyme to a secondary antibody generates a reagent that overcomes steric interference and provides enhanced accessibility to its target. The result is outstanding sensitivity and signal intensity.
Another feature of the ImmPRESS reagents is low background staining. Elimination of a large backbone allows better diffusion of the reagents to the target sites and a reduction in non-specific binding. The ImmPRESS Anti-Moouse Ig Reagent and the ImmPRESS Anti-Rabbit Ig (or Anti-Mouse Ig) Reagent are composed of our ultra-pure affinity-purified secondary antibodies coupled with a unique "micropolymer" of a very active form of peroxidase.
The ImmPRESS reagents are supplied prediluted in a convenient dropper bottle format. No mixing or titering of the ImmPRESS reagents is necessary to obtain optimal tissue section staining. The ready-to-use reagents along with fewer steps in the staining procedure should save time without sacrificing intensity or compromising background quality.
ImmPRESS™ Reagent kits consist of 50 ml of 2.5% normal horse blocking serum and 50 ml of the ready-to-use ImmPRESS™ Reagent.
Staining Procedure:
The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
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Multiple Antigen Labeling:
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ImmPRESS™ Reagents can also be used for localizing two antigens in the same tissues sections, even using two antibodies of the same species. After one antigen has been reacted with the first primary antibody and localized with ImmPRESS™ Reagent and a peroxidase substrate, the tissue is blocked once again with diluted normal horse serum. The second primary antibody is now added, followed by incubation in the appropriate ImmPRESS™ Reagent. A different peroxidase substrate is then used to identify the location of the second antigen. By choosing peroxidase substrates that give the highest contrast and selecting the order of staining the two antigens, multiple labeling in the same tissue section using ImmPRESS™ reagents can be easily achieved.
A complete printed brochure, "Discovery through color: A Guide to Multiple Antigen Labeling", is available upon request or online in pdf format from the link below. (Please note: this file is 8.5 MB).
For more details, please contact us at :
(33) 01 40 03 89 14
infos@abcysonline.com
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